Inhibitory Effect of TGF-/32 in Human Aqueous Humor on Bovine Lens Epithelial Cell Proliferation
نویسندگان
چکیده
Methods. Bovine lens epithelial cells (BLECs) obtained from the central zone of the anterior capsule were cultured in complete medium (F-12 nutrient mixture supplemented with 5% fetal bovine serum). Human aqueous humor was obtained from 11 patients during cataract surgery. Medium was removed and replaced with medium containing various concentrations of porcine platelet-derived TGF-/?2 or 10% human aqueous humor, with or without anti-TGF/?2 neutralizing antibody. The number of cells was counted at 96 hours.
منابع مشابه
Inhibitory effect of TGF-beta 2 in human aqueous humor on bovine lens epithelial cell proliferation.
PURPOSE To determine whether transforming growth factor-beta 2 (TGF-beta 2) in human aqueous humor inhibits the proliferation of lens epithelial cells. METHODS Bovine lens epithelial cells (BLECs) obtained from the central zone of the anterior capsule were cultured in complete medium (F-12 nutrient mixture supplemented with 5% fetal bovine serum). Human aqueous humor was obtained from 11 pati...
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